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Jay Mone' jmone at MARAUDER.MILLERSV.EDU
Fri Oct 2 09:02:00 EST 1998


A protein with an isoelectric point of 9 will have a net positive 
charge at pH 7-8.  If anything, it will float out of the top of the 
well.  You need to change to a buffer with a pH above the pI in order 
to impart a net negative charge.

Jay M.



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