high voltage vs. low voltage in gel electrophoresis

danwong at hongkong.com danwong at hongkong.com
Sun Oct 4 17:06:14 EST 1998


I am running some PCR products in  0.5% agarose and 0.75% Synergel.  The DNA
fragments range from 0.4 kb to 23kb - most of them are larger than 1kb.  The
gel looks good when it was run at 120 volts. However, a smear was seen at the
high molecular bands when it was run at 48volts. Does anyone know why and how
to deal with it? Thank you very much!


Daniel

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