Basic Q's for Molecular Biology
rhall at uvi.edu
Wed Apr 14 13:05:58 EST 1999
All good questions.
Standard questions all.
You will learn much as you work them out.
Enjoy the experience.
At 3:53 PM +0000 4/14/99, Jin-wook B. Paeng wrote:
>1. Why is DNA more stable than RNA?
>2. What would happen if we put DNA duplex into pure distilled water?
>3. Why is thymine used in DNA instead of uracil?
>4. The whole E. coli genome is about 4.6 Mbp. How many copies of it
>are in 1 micro gram
>of DNA sample?
>5. Promoter is the region located at the 5' end of genes. It is
>recognized by RNA
>polymerases and the recognition is the most important step for the
>initiation of gene
>expression. If we assume there are one thousand promoters with an
>identical sequence in E.
>coli genome, what should be the minimum length of this promoter
>sequence, assuming that
>RNA polymerases recognize bases as we read alphabets?
>6. What is the average size of the DNA fragments that are obtained
>from the full digestion
>by 2 different restriction enzymes in a single reaction mixture, in
>which one is 6 base and
>the other one is 4 base recognition?
>7. The communication of information in life is done by the
>complementarity of molecules.
>Protein sequence is determined by mRNA sequence, which is in turn
>copied from DNA
>sequence. We call this flow of information from DNA to RNA to protein
>as the central
>dogma of life. RNA can easily be copied from DNA by the molecular
>they use the same complementary pairs of bases. (The difference
>between uracil and thyme
>does not hinder the complementarity.) In contrast, protein has no such
>complementarity as in between DNA and RNA. Then, how can the
>information in protein
>sequence be copied from mRNA? What is the key step in this
>8. Transcription is the process of making RNA from DNA. (Translation
>is making protein
>from mRNA.) Chromosomes are either closed circles (for eubacteria and
>very long linear strings (for eukaryotes). What would happen
>physically on these
>chromosomes while a region within them is being transcribed?
>9. What is the fundamental reason that we can only separate DNA
>fragments of about one
>thousand bases or so at the very best using the current sequencing
>10. What is the most important discovery done by James D. Watson for
>the elucidation of
Richard Hall, Associate Professor of
Comparative Animal Physiology
Division of Sciences and Mathematics
University of the Virgin Islands
St. Thomas, USVI 00802
rhall at uvi.edu
More information about the Bioforum