Help - COS Cells..

Nick Theodorakis theodorn at gusun.georgetown.edu
Fri Apr 23 16:30:01 EST 1999


In article <bpmurray*STUFFER*-2104991437590001 at mac-daddy.ucsf.edu>,
  bpmurray*STUFFER*@socrates.ucsf.edu (Bernard P. Murray, PhD) wrote:
<snip>
>
> The odd thing is that some groups have maintained separate "1" and
> "7" cultures for so long that there are now some minor phenotypic
> differences between them so that you could probably see differences
> in transfection efficiency...
<snip>

That's certainly easy to believe. When I was a grad. student, we used to keep
two kinds of HeLa cells (and I don't mean two established "strains" like S3
and JW36 - these were supposed to be both JW36) - one for transfection, and
one for Adenoviral infection - because "our" cells didn't transfect well, but
cells with a different lab "pedigree" did transfect well.

--
 _______________________________________________
| Nick Theodorakis                              |
| theodorn at gusun.georgetown.edu                 |
|_______________________________________________|

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