Using Zeocin in slection after transfection !!

boella boella at ust.hk
Mon Feb 8 22:02:15 EST 1999


Dear all,

I am now doing cell transfection of a mammalian expression vector
pSecTag B (Invitrogen) to a mouse fibroblast cell line L929. Since the
vector contains a Zeocin selection marker, I would like to know what
will be the minimal concentration of Zeocin in the selection medium to
kill untransfected L929 cells.

Thank you for your kindest help and suggestion.

Regards,
Ella Chan
HKUST
boella at ust.hk




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