Using Zeocin in slection after transfection !!

boella boella at
Mon Feb 8 22:02:15 EST 1999

Dear all,

I am now doing cell transfection of a mammalian expression vector
pSecTag B (Invitrogen) to a mouse fibroblast cell line L929. Since the
vector contains a Zeocin selection marker, I would like to know what
will be the minimal concentration of Zeocin in the selection medium to
kill untransfected L929 cells.

Thank you for your kindest help and suggestion.

Ella Chan
boella at

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