5'RACE problem

Bernard P. Murray, PhD bpmurray*STUFFER* at socrates.ucsf.edu
Wed Mar 3 20:02:24 EST 1999


In article <38156025.1.5643 at mx1-11.onmedia.com>, "KevinKao"
<KevinKao at goplay.com> wrote:

> Hi, I am encountering a problem trying to deduce the 5' end sequence 
> of a parainfluenze virus.  I have tried the Clonetech kit and didn't 
> obtained a good result.  Then I checked the published sequence of PIV 
> and found that there might be some secondary sturctures that could 
> interfere with my RT reaction.  I have also tried the T4 RNA ligase 
> method with a short piece of DNA oligo (I am still waiting on my RNA 
> oligo) but it didn't work possibly due to the low efficiency of the 
> RNA ligase working with a DNA oligo.  
> 
> So my question is that has anybody out there who can give me some 
> pointer on how they did it???  Or some information on where I could 
> find some good literature???  Thanks 
> 
> Kevin  


bionet.general is dead as far as bench science is concerned.

Try posting to bionet.molbio.methds-reagnts (hopefully my
newsreader will have allowed me to to this for you).
Sorry I can't help you myself but I didn't want to leave
you stranded.
     Bernard
-- 
Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA



More information about the Bioforum mailing list