Cell Line Establisment

[Pierre MINTZ]

you can try to irradiate normal fibroblasts to use them only as a feeder

Jason Tokunaga wrote:

> I am trying to establish primary cell lines from head and neck tumors. I
> seem to
> be able to grow them, but I can't seem to be able to passage them
> without the
> normal fibroblasts from overgrowing the culture and ruining my chances
> of
> growing the head and neck tumor.   Any comments.  Thx.