Cell Line Establisment

Pierre MINTZ mintz at lbpa.ens-cachan.fr
Tue Nov 23 10:41:24 EST 1999

you can try to irradiate normal fibroblasts to use them only as a feeder

Jason Tokunaga wrote:

> I am trying to establish primary cell lines from head and neck tumors. I
> seem to
> be able to grow them, but I can't seem to be able to passage them
> without the
> normal fibroblasts from overgrowing the culture and ruining my chances
> of
> growing the head and neck tumor.   Any comments.  Thx.

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