ProteinASepharose Purification of Antibodies

[Jens Lohrmann]

Dear Netters!

Just a short question:
Can you please send me or quote an easy and reliable protocol for
proteinAsepharose purification of antibodies? Especially I'm wondering
if there are alternatives to the acidic elution or how long you've got
to  incubate the sepharose/antibody with the acid.

Thanks a lot!
Sincerely
Jens


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Jens Lohrmann

lohrmann at sun2.ruf.uni-freiburg.de

http://www.biologie.uni-freiburg.de/data/schaefer/jl01.html
http://www.biologie.uni-freiburg.de/data/schaefer/jl02.html
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Schänzlestr. 1
Institut für Biologie II / Botanik
79104 Freiburg
Fon: 0761/203-2619
Fax: 0761/203-2612
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