Sequencing AT rich template
hsuyc99 at libra.seed.net.tw
Tue Apr 4 08:28:58 EST 2000
I have a trouble PCR template recently. This template is about 400 bp. The sequencing data show about 98% of A and T in this template. The worse thing is there exist a stretch of about 20 Ts following about 20 As. The slippage is a problem to this template, no matter from farward or reverse. Both direction show the same result. Although I can assemble the whole sequence from farward and reverse result. I can not comfirm the exact number of A and T. Any one experience this kind of template? Or, any sequencing kit can resolve this problem. Thank you very much.
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