Labelling a neurotransmitter

Robert M. Mihalek mihalek at FORMULA1.smtp.anes.upmc.edu
Mon Feb 7 17:03:57 EST 2000


In article <87nf07$cgd$1 at usenet01.srv.cis.pitt.edu>
"Terry Morris" <tjm5+ at pitt.edu> writes:

> Please, can anyone advise or suggest alternative approaches.
> 
> I am investigating the effects of general anesthetics on the fast transport
> of neurotransmitter containing vesicles in axons and the pre-synapse. Is
> there a way to fluorescently label either the neurotransmitter (glutamate)
> or some component of vesicular membrane so that active transport can be
> recorded in real-time using confocal microscopy?
> 
> Alternatively, is it possible to label the ATP turnover of the molecular
> motor (kinesin) driving vesicle movement in vivo?
> 
> Terry Morris

Dear Terry, 

You may want to look into using a dye called FM1-43. I know this can be
used to fill vesicles, and then various aspects of vesicle turnover can
be studied using microscopy. Look up papers by Tim Ryan to get an idea
how it's done.


Bob Mihalek=================================================
please remove the name of the RACE SERIES for e-mail replies
==========================================================
"They came to our village a year ago and were looking only for
men with guns. A lot of people didn't believe they would kill
the women and children." Hamdi Fazliu - Kosovo High School
The Boston Globe July 4, 1999

It is the human moral will that saves us from violence, not the
presence or absence of weapons. We should reject utterly the absurd
theory
that weapons are the cause of violence.  ALAN KEYES






More information about the Bioforum mailing list