How to generate a precise 3' adn 5' ends of RNA

ephray ephray at home.com
Sun Feb 20 15:36:55 EST 2000


Fosr most of the commercially available transcription vector, it is
generally possbile to generate a precise 5' end of the RNA.  The 3' end
however is normally dictated by the presence of polyA signal which in
turns lead to an addition of poly A tail in transfected cells.  The
question is: if I want to generate a RNA with a precise 3'end in a
trasnfected cells (no polyA tail etc.)  is there a way to do that?

Thansk in advance.


Anita
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