Sea sponges - DNA extraction protocol

Jérôme Rigot jerome at chemistry.ucsc.edu
Fri Jan 21 13:44:09 EST 2000


Hello,

We are working with sea sponges, and right now we would like to extract
DNA from these organisms'tissues.  

We have been following standard protocols (including phenol chloroform
extraction), but we have had problems with various contaminants, maybe
from pigments or/and humic acids.  

If anybody has had experience with DNA extraction from sea sponges and
would be willing to share it with us, it would be great.  Any help would
be welcome.

Thanks, 

Jerome Rigot
Dept. of Chemistry and Biology
Marine Natural Products
University of California,
Santa Cruz, CA 95060

email: jerome at chemistry.ucsc.edu
(831)459-5641




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