Aggregation of IgG

spam_out at zfree.net spam_out at zfree.net
Mon Jul 3 02:46:27 EST 2000


You could try titrating immediately out of the pH 3 conditions. This can be
done by eluting directly into a "strong" Tris buffer.

Tarmo Humppi <tarmo.humppi at pvtt.mil.fi> wrote:
> We have tried to purify polyclonal rabbit antibodies from serum using
>affinity column that has the peptide antigen attached to it. The
>problem is that the eluted IgG aggregates immediately after elution. We
>have used Tris buffer pH 7.5 as starting buffer and glysine pH 3.0 for
>elution. Is there a way to get the aggregated IgG back to solution? We
>have tried increasing ionic strenght but it hasn´t helped. All tips are
>appreciated!
>
>--
>Tarmo
>
>
>
>
>
>
>Sent via Deja.com http://www.deja.com/
>Before you buy.



http://www.zfree.co.nz

Brian Aitken

baitken at zfree . co . nz






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