Fixation of bone/cartilage material

[Yvonne Mueller]

Hi!

I want to make ultrasections of complete toes of amphibia.
We use Spurr to embed the material.
Do you have any ideas how to fix the amphibian material and how to decalcify?
We used glutaraldehyd and EDTA, but that does not seem to work to well ...

Thanks!
-- 
* Yvonne Mueller
* Email: muellery at uni-duesseldorf.de
* WWW: http://www-public.rz.uni-duesseldorf.de/~muellery