Protein separation via ion-exchange chromatography

mp candirectory at yahoo.com
Fri Jul 14 23:20:26 EST 2000


Hi
DEAE has a high capacity for proteins, so a column of 15x 2 cm  should
be ok

Mahmood  mpiraee at is2.dal.ca

Brett wrote:

> I intend to separate some proteins by DEAE sephadex
> ion-excheange chromatography.  My concern is this:
>
> I have not found any theory regarding the relationship
> between the concentration of the sample to be applied
> to the column and the dimensions of the column.
>
> My sample is quite dilute (~0.35 mg/ml total protein in
> about 9 ml). Can anyone give me some insight into the
> appropriate column size I should be using?  Does the
> sample concentration matter?
>
> As an aside, I am wondering how many people actually
> read these bionet newsgroups since they tend to be quite
> empty.  Is this because people read but don't post, or
> because the answers to the questions that are asked are
> too obscure for those who do look through bionet??
> I'm just concerned that I'm throwing my question out to
> only about 10 people or something.... So if anyone knows
> of a place I might be more likely to get a response, that
> information would also be much appreciated.
>
> Thanks in advance.
>
> Brett O'Connell
> Muscle Cell Biochemistry Lab
> Victoria University
> Melbourne, Australia
> Ph   +61-03-9688-4785
> Fax +61-03-9688-4995






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