Incubation buffer

Jim Mensch jm68 at midway.uchicago.edu
Wed Jun 21 14:51:53 EST 2000


The process described will yield a final concentration less than 10mM
NaPO4.  Make a 10x (100mM) solution of NaPO4, use an aliquot of this
to make a preliminary solution of about 75% the desired final volume,
adjust to the desired pH with a concentrated (1 M perhaps) solution
of citric acid, then add water to the final volume.  Re-check pH and
adjust after the solution has stood a while ( dilution and temperature
shifts may affect the final pH).  Calbiochem used to distribute a great
little pamphlet on buffer preparation and theory.  Hope this helps.

-- 
 
Jim Mensch <j-mensch at uchicago.edu> 
We are confronted with insurmountable opportunities.- Walt Kelly, "Pogo"






More information about the Bioforum mailing list