protein which stops translation

Carlos Ribeiro Carlos.Ribeiro at unibas.ch
Wed Jun 28 03:40:53 EST 2000


Hello,

I want to use it in Drosophila embryos. So don't worry about the
induction. I will simply use the UAS/GAL4 system. I just need an idea as
to which protein I should induce which could stop translation.

Thanx for the suggestions,

Saludos,

Carlitos

Nick Theodorakis wrote:
> 
> Carlos Ribeiro <Carlos.Ribeiro at unibas.ch> wrote:
> >Hello out there!
> >
> >Does anyone know of a protein which stops translation and which
> could be
> >used to stop translation by inducing it? As an alternative to
> >cyclohexamyde!
> >
> 
> It depends on what you mean by "inducing" it.
> 
> Ricin inhibits translation by modifying rRNA.
> 
> There's a poliovirus protein (don't remember the name, sorry)
> that
> inactivates the cap-binding protein. Does viral infection count
> as
> "inducing?"
> 
> I suppose if you can generate a constitutively active eIF2 kinase
> that might work, too.
> 
> Nick Theodorakis
> 
> nicholas_theodorakis at urmc.rochester.edu
> Got questions?  Get answers over the phone at Keen.com.
> Up to 100 minutes free!
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Carlos Ribeiro                         
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