Help: HeLa cell culture

Yap Wen Jiun wenjiun at yahoo.com
Sun Mar 12 02:26:49 EST 2000


Why is the monolayer of a HeLa cell culture not formed eventhough it is
not contaminated? The cell concentration is 500000 sel/mL and is in 10
mL with RMPI-1640 plus 10% serum, penicillin and streptomycin in culture
flask 25 cm2. Why is the cell layer not formed and how to overcome this
problem?




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