How to quantify antisens mRNA

[Frank Ludewig]

You can distinguish just by choosing "differential" primers, for that you
shouldn`t have taken the complete cDNA for antisense expression, or (if you
want to "quantify" your transgene) choose primers sitting on the MCS of your
vector.
FrankL


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~~~~~~~~~~~~~~~~~~~~~~~!

Dr. Frank Ludewig
Department of Botany II
University of Cologne
Gyrhofstrasse 15
50931 Cologne
Germany

email: frank.ludewig at uni-koeln.de
Fax:   49-(0)221-4705039
Phone: 49-(0)221-4703388

http://www.uni-koeln.de/math-nat-fak/botanik/bot2/agflue/




David Humair <david.humair at rac.admin.ch> schrieb in im Newsbeitrag:
3A029879.F855B75 at rac.admin.ch...
Hi all,
I have a theoretical problem with RTPCR on antisens mRNA. How can I
distinguish between the antisens mRNA and the endogenous sens mRNA?

Thanks for your help

--
Dr. David Humair
Station fédérale de recherches en production végétale de Changins
Laboratoire de génie génétique
Route de Duiller
CH-1260 Nyon

Tél : +41-22-3634419
Email : David.Humair at rac.admin.ch