Cloning in serum free media (limiting dilution)

sarit zegenrich saritz at
Tue Nov 21 20:18:36 EST 2000

I am interested in cloning (limiting dilution) in serum free media (SFM)
What is the best media recomended?
Do you have a recomended protocol?
Do you use fresh medium or "conditioned" medium?
Do you use "adapted" cells or cells after transfection?


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