huihuang at iupui.edu
Wed Apr 18 14:14:47 EST 2001
After aminoethylation of polyacrylamide(bio-gel p100),
I wash 1g this amine-containing matrix with water and then with 0.1M
sodium phosphate, 0.15M NaCl, pH 7.0 (coupling buffer). Drain to a
moist cake.And then add 100ml 12.5% glutaraldehyde (w/v) in coupling
buffer to the gel. Again add 0.6 gm sodium cyanoborohydride (toxic;
use a fume hood) or 0.5g sodium borohydride to the gel and mix for
at least 4hr. But when I add glutaraldehyde to gel(matrix),the gel'colour
change from white to yellow immediately,and become more and more deeper,
become brownish black at the end of reaction.
Is it normal?
Thanks in advance!
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