bata-actin and mitochondria

hulai zai zaihulai at
Thu Jan 25 22:30:39 EST 2001

When you fractionate mitochondria from cytosol, and do
Western using anti-b-actin antibody, would you expect
to see b-actin band in mitochondrial fraction.  I did
once, when I use anti-b-actin to check loading of
cytosol fraction, I also found b-actin in
mitochondrial fraction, is this normal ?  thanks. 
please e-mail me at  zaihulai at

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