Purifying small volumes of antibodies
Mark.Haynes at Mail.TJU.Edu
Wed Jan 31 15:15:51 EST 2001
why not use a column
or if available hplc or something of that sort?
Nanci E Donacki wrote:
> I am looking for some suggestions to purifiy small volumes (~2 ml supe) of
> monoclonal antibodies. I've found filter plates from several manufacturers
> for DNA purifications, but not proteins.
> I am currently looking at using filter plates with small amounts of protein
> G, then eluting and collecting into 96 well plates. I'm concerned that I'll
> lose more than recover.
> Any suggestions?
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