Purifying small volumes of antibodies

Mark Haynes Mark.Haynes at Mail.TJU.Edu
Wed Jan 31 15:15:51 EST 2001


why not use a column
or if available hplc or something of that sort?


Nanci E Donacki wrote:

> I am looking for some suggestions to purifiy small volumes (~2 ml supe) of
> monoclonal antibodies.  I've found filter plates from several manufacturers
> for DNA purifications, but not proteins.
> I am currently looking at using filter plates with small amounts of protein
> G, then eluting and collecting into 96 well plates.  I'm concerned that I'll
> lose more than recover.
> Any suggestions?
>
> Nanci






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