culture contamination

paul.fonteneau fonteno at pharma.u-strasbg.fr
Wed Mar 28 02:52:05 EST 2001


maya wrote:
> Under light microscopy dark dots are visible in the cells and are likely
> to be also moving on their surface. Cell growth, attachment and
> viability are affected.

I had the same thing a few years ago.

> These mysterious creatures are only observed in association with cells
> but not in incubated cell free medium.

Well...

> If you have an idea who these intruders could be or have an advice how
> to recognize them or get rid of them please help me.

Hypothesis : mycoplasma (?)
Test : Hoechst staining
Remove : passage in vivo (a few days in the peritoneum of mouse...)




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