detection of small differences in dna fragment sizes

Latch, Emily emily at fnr.purdue.edu
Sat Sep 1 15:26:37 EST 2001


We have been using 4% Trevigel.  It can resolve fragments (we are usually
dealing with PCR products of about 100-300bp) that differ by about 5bp (2-3
if you look really closely).  Haven't tried running higher % Trevigel, but
it is difficult enough to get it into solution as it is (need to boil it for
about 5-6 minutes).

Emily Latch, Purdue University

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