2D: spot disappearance
anne-francoise.marchand at umh.ac.be
Fri Aug 2 03:14:11 EST 2002
Two years ago, I have done about 50 2D gels (silver staining +
glutaraldehyde). After analyse, we have fond one very interesting
spot. Thus we decided to do the same maps (same protocol) with the same
sample frozen at -80°C. The spot had disappeard!
How can you explain that? Do you have any solution to find again the
spot? Do you have a protocol to extract a spot from a silver staining
gel with glut. (I shoud analysed the spot with a Q-TOF)? How can I know
the name of this spot?
Thanks for your ideas
anne-francoise at umh.ac.be
-------------- next part --------------
A non-text attachment was scrubbed...
Size: 338 bytes
Desc: Carte pour Anne-Fran?oise Marchand
Url : http://iubio.bio.indiana.edu/bionet/mm/bioforum/attachments/20020802/a49ee36c/anne-francoise.marchand.bin
More information about the Bioforum