Protein quantification in 8M urea - any suggestions?

Myers James J James.Myers at avecia.com
Fri Aug 2 10:10:46 EST 2002


I need a simple way of determining the concentration of a protein in 8M urea
(dissolved inclusion bodies). I've tried absorbance at 280nm but there seem
to be too many impurities. 

I'd be grateful for any suggestions!

regards
James Myers
Postgrad Student
UCL Biochemical Engineering and Avecia Biologics.

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