Protein Expression

Peijun ZUO s003206 at mailserv.cuhk.edu.hk
Sat Jan 25 09:14:51 EST 2003


Dear Scientists,

    I cloned 12 genes in a plasmid with lac promoter and lac operator, and
transformed in DH5£\. Two of them expressed more than 70% in the soluble
protein. Others were vey bad. All of the bad genes have at least 6 base
pairs (and in frame) identical or complement with the operator sequence. The
good two have not (they have also but not in frame).

    Would you can explain why?

    Thank you very much!

    Your Sincerely

Peijun





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