[Bioforum] qPCR NEWS March 2008 - qPCR robotics

Editor www.Gene-Quantification.info via bioforum%40net.bio.net (by editor from gene-quantification.info)
Tue Mar 25 09:07:14 EST 2008

qPCR NEWS March 2008 - qPCR robotics

Dear researcher,
dear Gene Quantification page reader,

Our newsletter informs about the latest news in quantitative real-time
PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene
Quantification homepage. The focus of this newsletter issue is:

- New - qPCR robotics
- Update - RNA interference & small interfering RNA  (siRNA)
- qPCR application workshops in 2008
- If this newsletter is not displayed correctly by your email client,
please use following LINK:  http://qpcrnews.gene-quantification.info/

qPCR robotics - http://qPCRrobotics.gene-quantification.info

Real-time quantitative PCR (qPCR) is now a mainstay of molecular
biology. Just a few short years ago the purchase of a real-time
instrument was considered a luxury. Now most laboratories either
posses a real-time instrument or have easy access to one. We predict
the same will happen for robotic automation of qPCR setup; it will
soon be commonplace and probably expected.

A trend to automation is easy to understand. Firstly, the sheer
numbers of reactions that are run (all with one or more replicates)
means the workload is increasing dramatically. Secondly, and more
importantly, qPCR setup requires great skill and lots of practice
because even a very small variation when pipetting a DNA or RNA sample
translates into big differences after amplification. There is also the
question of repeatability. Identical results from different operators
and between laboratories are expected but that can be very hard to
achieve. Let's not forget that setting up qPCR assays is also tedious
and time consuming! A small and affordable liquid handling robot with
the precision necessary to tackle qPCR setup would address all these
issues. The good news is robots like this are now available.

Choosing a robot - the essential attributes
The importance of robot software
qPCR applications using pipetting robots
Movie:  PCR technology in BVDV diagnostics  /  molecular
diagnostics  /  LIMS

... read more on ... http://qPCRrobotics.gene-quantification.info



Update - RNA interference (RNAi):  siRNA,  saRNA  and  microRNA
A lot of new RNAinterference papers using siRNA, saRNA and microRNA
applications are present on various sub-pages.

RNA interference (RNAi) is a mechanism that inhibits or activates gene
expression at the stage of translation or by hindering the
transcription of specific genes. RNAi targets include RNA from viruses
and transposons (a form of innate immune response), and also plays a
role in regulating development and genome maintenance. Small
interfering RNA strands (siRNA) are key to the RNAi process, and have
complementary nucleotide sequences to the targeted RNA strand.
Specific RNAi pathway proteins are guided by the siRNA to the targeted
messenger RNA (mRNA), where they "cleave" the target, breaking it down
into smaller portions that can no longer be translated into protein. A
type of RNA transcribed from the genome itself, microRNA (miRNA),
works in the same way.

RNA interference: hitting the ON switch.  Researchers in San Francisco
have findings that suggest a whole new side to RNA interference. Erika
Check reports on their attempts to make a revolutionary field more
revolutionary still.

Molecular biology: The expanding world of small RNAs
Molecular cell biology has long been dominated by a protein-centric
view. But the emergence of small, non-coding RNAs challenges this
perception. These plentiful RNAs regulate gene expression at different
levels, and have essential roles in health and disease.

... read more on ... http://RNAinterference.gene-quantification.info/


update - microRNA

A lot of new papers and database links around microRNA are present on
5 pages.



With the new qPCR INFO PORTAL and all the presented tools we will help
you with to find the right information about qPCR and related topics
in Molecular Biology in the literature and in the World Wide Web.
=>  Papers / Protocols / Methods / Databases / Alets / Feeds / Books /
Forums / E-mail / Directory



Upcoming Events World-wide academic and commercial qPCR Events

Symposia, Meetings, Conferences, Workshops, Seminars, Online-Seminars,
qPCR Education Program, ...etc...
Please submit your qPCR event here  =>  events from gene-



TATAA Biocenter Germany - qPCR Application workshops

At the TATAA Biocenter Germany we offer qPCR application workshops,
the 3-day Core Module and a 2-day Biostatistics Module.  qPCR courses
are held in regularly in Göteborg, Sweden, in English and in Freising-
Weihenstephan, Germany, in German and English, and in Prague, Czech
Republic in English and Czech.
Depending on the occasion the workshop language and the different
prices may apply. Further customized workshops and specialized
trainings will be held as well across Europe and world-wide. TATAA
Biocenter Germany courses are held in cooperation with the Institute
of Physiology, located at the Technical University of Munich, in
Freising-Weihenstephan, near Munich, very close to the Munich Airport
(MUC). For more information and to register for the qPCR application
workshops, please see our web page:  http://tataa.gene-quantification.info/

Course Occasions 2008:
3-day qPCR Core Module  (Mon. - Wed.)    and     2-day BioStatistics
Module (Thu. - Fri.)

*   5 - 9th May 2008 (in Freising, Germany, Kurs wird in DEUTSCH
gehalten, German language)
*   7 - 11th July 2008  (in Freising, Germany, English language)

Please register here  =>  http://www.tataa.com/Courses/Courses.html


Forward Please send the qPCR NEWS to further scientists and friends
who are interested in qPCR !

Best regards,

Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages


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