[Bioforum] biotinylated PCR stability

Marcelo Viegas via bioforum%40net.bio.net (by mhviegas from yahoo.com)
Sun Jul 5 13:57:21 EST 2009

I am doing PCRs using biotin-dCTP. Afterwards, I keep the biotinylated product in the fridge. I noticed that if I use the labeled PCR the following day for my experiments, I get high signals (streptavidin development) but if I use it 3-4 days later instead, the signal is very weak. Does anyone know whether the biotin is that unstable bound to DNA? Thanks in advance.

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