[Bioforum] Jurkat transient transfection protocol

Andrew Suh via bioforum%40net.bio.net (by neoret from msn.com)
Fri Sep 3 03:25:01 EST 2010


Hi everyone,

I am trying to transfect some Jurkat cells using the DEAE-dextran method.

I am only getting abou5 2-5% transfection efficiency. I was wondering if anyone can send me a protocol that gives a lot better transfection efficiency for this cell line. Please either reply to this forum of email me neoret at msn dot com. 

Thanks in advance for your help.

David
 		 	   		  


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