[Bioforum] Re: Tricine gels
Dr Engelbert Buxbaum
(by engelbert_buxbaum from hotmail.com)
Thu Sep 30 17:50:54 EST 2010
In article <b11e3d17-d3f6-40b8-9e44-
e1fca883fc0e from q40g2000prg.googlegroups.com>, aannamma from gmail.com says...
> We have been trying to do tricine gels and have been running the gels
> at 90V... However we have observed extensive heating up of the gel
It is usually more effective to run gels at constant current, 10-20 mA
per small (8x6 cm^2) gel. You may also use a cooled system, e.g.,
Other than that, keep conductivity as low as possible. Some heating is,
however, inevitable. If you run a gel at 20 mA, the voltage will
increase during the run from 60 to 200 V, that's up to 4 W powered into
some 2 ml gel volume for 1 h or so.
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