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Hello fellow slaves of science.<br>I'm trying to create a pspA reporter plasmid by linking the psp
promoter using the thi- genotype of E.coli MC4100 as an auxotrophic
marker, however I'm having 'a little' trouble finding out which gene
has actually been deleted or mutated so that I can incorporate it into
my plasmid.<br>Does anyone out there know what gene the thi- in E.coli MC4100 actually is?<br>Thanks,<br>Julian<br><br /><hr />Find a cure at MSN NZ Money. <a href='http://money.msn.co.nz' target='_new'>Got a 2008 financial hangover?</a></body>
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