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Hello everybody,<BR> <BR>here it is my first question. I am using Ni-NTA agarose beads to purify a protein.<BR>According to the manufacturer the limit pressure is around 2 psi (nothing), and I am running the column to 50 psi.<BR> <BR>Can anyone please tell me about why does the pressure limit is important when dealing with affinity chomatography?<BR> <BR>How can this (pressure limit too high) affect my purification?<BR> <BR>Am I destroying the beads?<BR> <BR>I am running the column (home-prepacked tricorn) on an akta-prime.<BR> <BR>I would really appreciate any information since I couldn't find any info on the web.<BR> <BR>Thank you very much,<BR> <BR>Eva<BR><br /><hr />Messenger cumple 10 años <a href='http://www.vivelive.com/aniversariomessenger' target='_new'>¡Conéctate y celébralo con toda la comunidad!</a></body>
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