Pradeep M Joshi
pad29 at ncbs.tifrbng.res.in
Mon Feb 10 04:46:15 EST 1997
I am a new subscriber to the news group. I have run into a small problem.
I want to do some surface staining of HaCat, an epithelial cell line for
a FACS scan. The Problem now is that my cells dissociate only in th
presence of trypsin and consequently most of my cell surface markers ,
receptors get chewed up. could you suggest me some way of getting a
single cell suspension with my cell surface molecules intact for a FACS scan.
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