Fall courses at Cold Spring Harbor Lab

CSHL theiss at cshl.org
Wed Mar 10 12:54:23 EST 1993



                  The following COURSES are being held
                             in 1993 at
                     COLD SPRING HARBOR LABORATORY 



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     Applications and information can be obtained from:
         COURSE REGISTRAR
         Cold Spring Harbor Laboratory
         1 Bungtown Road
         Cold Spring Harbor, NY 11724-2213
              Phone: (516) 367-8345
              Internet: meetings at cshl.org
     ============================================================



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MACROMOLECULAR CRYSTALLOGRAPHY

October 13 - 26

William Furey, V. A. Medical Center, Pittsburgh
Gary Gilliland, Center for Advanced Research in Biotechnology
Alexander McPherson, University of California, Riverside
Jim Pflugrath, Cold Spring Harbor Laboratory

Crystallography and X-ray diffraction yield a wealth of structural
information unobtainable through other methods.  This intensive
laboratory/computational course will focus on the major techniques used to
determine the 3-dimensional structures of macromolecules.  It is designed
for scientists with a working knowledge of protein structure and function,
but who are new to macromolecular crystallography.  Topics that will be
covered include: protein purification, crystallization, crystal
characterization, data collection, data reduction, anomalous dispersion,
phase determination, molecular replacement and averaging, electron density
interpretation, structure refinement, molecular graphics, and molecular
dynamics.  Participants will learn through extensive hands-on experiments,
informal discussions and lectures on current applications of these and
related procedures given by outside speakers.


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ADVANCED IN SITU HYBRIDIZATION AND IMMUNOCYTOCHEMISTRY

October 13 - 26

Paul Hough, Brookhaven National Laboratory
Ken Jacobson, University of North Carolina, Chapel Hill
David Spector, Cold Spring Harbor Laboratory
Barbara Trask, University of Washington School of Medicine, Seattle

This course focuses on specialized techniques and concepts in microscopy
related to localizing nucleic acid sequences and proteins in cells and
preparing isolated nucleic acids for microscopic examination.  The aims of
the course are designed to provide state-of-the art technology and
scientific expertise in the use of microscopic applications to address
basic questions in genome organization and cellular and molecular biology.
The course is designed for the molecular biologist who is in need of
microscopic cell biological approaches and for the cell biologist who is
not familiar with the practical application of the advanced techniques
presented in the course.  The course will emphasize the use of the latest
equipment and techniques in epifluorescence microscopy, confocal laser
scanning microscopy, and electron microscopy to localize nucleic acids and
proteins in mammalian cells.  Among the methods presented will be
preparation of tagged nucleic acid probes, fixation methods, detection of
multiple nucleic acids in a single cell, chromosome spreads, use of a
variety of reporter molecules and non-antibody fluorescent tags, direct
and indirect antibody labeling, detection of multiple proteins in a single
cell.  In addition, techniques to observe isolated nucleic acids and
proteins will be presented.  In each method, several experimental
protocols will be presented allowing the students to assess the relative
merits of each and to relate them to their own research.  The laboratory
portion of the course will be supplemented by invited lecturers who will
give up-to-the minute reports on current research using the techniques
being presented in the course.


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ANALYSIS & GENETIC MANIPULATION OF YEAST ARTIFICIAL CHROMOSOMES

October 14 - 27

Eric Green, Washington University School of Medicine
Claire Huxley, St. Mary's Hospital Medical School, United Kingdom
Rodney Rothstein, Columbia University College P & S

Cloning in yeast artificial chromosomes (YACs) is rapidly being applied to
a wide variety of molecular genetic problems.  This course will provide
basic scientific expertise in current techniques for the analysis and
manipulation of YACs.   In general, a blend of theoretical and practical
information will be provided, with the goal to establish a strong
foundation for applying YAC cloning to a diversity of scientific problems.
Topics include standard yeast genetic techniques (such as the propagation
and storage of cells, tetrad dissection, colony hybridization, and DNA
transformation), YAC library screening using both PCR- and genetic-based
strategies, characterizing YAC inserts by pulsed-field gel electrophoresis
and PCR, manipulating YAC clones by recombination-mediated disruption,
targeted integration, and YAC-YAC recombination, and techniques for
introducing YACs into mammalian cells.  Participants will learn through
hands-on experience, informal discussions and lectures given by prominent
experts designed to complement the experimental activities.


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ESSENTIAL COMPUTATIONAL GENOMICS FOR BIOLOGISTS

November 3 - 12

Elbert Branscomb, Lawrence Livermore National Laboratory
Nat Goodman, Whitehead Institute & Massachusetts Institute of Technology
Eric Lander, Whitehead Institute & Massachusetts Institute of Technology
Thomas Marr, Cold Spring Harbor Laboratory
Eugene Myers, University of Arizona

This course is intended primarily for molecular biologists and geneticists
who are mounting large-scale projects that require informatics components.
No prior experience with the use of computers or mathematics is assumed.
Lectures and computer work will delve deeply into both the theoretical
issues and practical approaches to a number of important computational
problems in genomic analysis.  The course will include an overview of
informatics systems analysis and design principles.  Topics include such
basics as database design and data modeling to more complex analytical
methods.  Technical issues relating to the analysis of raw data such as
image analysis, sequence assembly, physical mapping, genetic-linkage
analysis, and data integration will be covered.  Students will be
introduced to, and will gain hands-on experience with, a variety of
software tools used in computer systems design and data acquisition and
analysis running on Macintosh computers.  It is intended that students
will gain sufficient knowledge and experience from this course to enable
them to initiate active collaborations with computational scientists at
their home institutions.


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MOLECULAR GENETICS, CELL BIOLOGY & CELL CYCLE OF FISSION YEAST

November 2 - 15

Tom Chappell, Duke University
Peter Fantes, University of Edinburgh
Maureen McLeod, State University of New York, Brooklyn

Recent advances in cell cycle research, largely due to studies on the
fission yeast Schizosaccharomyces pombe, have prompted much interest in
this organism.  While cell cycle studies continue to fluorish, S. pombe is
increasingly being chosen as a model organism for investigations into
other aspects of cell biology and genetics using the powerful molecular
and genetical techniques available.  The content of the course reflects
all these areas of interest, and will provide participants with the skills
necessary to pursue their own investigations.  Topics covered include
mutagenesis and analysis of mutants,transformation and gene transplacement
techniques, isolation of nuclei, preparation of nuclear DNA, plasmid
recovery from yeast into bacteria, cell cycle methods, cytology and
immunological techniques.  In addition to hands-on experience,
participants will have the opportunity to learn through informal group
discussions and formal lectures given by prominent S. pombe researchers
drawn from the expanding international community.


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MONOCLONAL ANTIBODIES FROM COMBINATORIAL LIBRARIES

November 2 - 15

Carlos Barbas, Scripps Research Institute
Dennis Burton, Scripps Research Institute

Recent advances in the generation and selection of antibodies from
combinatorial libraries allow for the rapid production of antibodies from
immune and non-immune sources.  This laboratory/lecture course will focus
on the construction of combinatorial antibody libraries expressed on the
surface of phage and selection of desired antibodies from the libary.
Students will learn the theoretical and practical aspects of constructing
combinatorial libraries from immune and non-immune sources as well as the
construction of synthetic antibody libraries.  Antibodies will be selected
from the library by panning.  Production, purification and
characterization of Fab fragments expressed in E. coli will also be
covered.  Students will also be encouraged to select antibodies against an
antigen of their interest from pre-existing libraries.  The lecture
series, presented by a number of invited speakers, will focus on PCR of
immunoglobulin genes, the biology of filamentous phage and the utility of
surface expression libraries, expression of antibodies in E. coli and
mammalian cells, antibody structure and function, catalytic antibodies,
and recent results on the use of antibodies in therapy.


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MOLECULAR MARKERS FOR PLANT GENETICS & PLANT BREEDING

November 2 - 15

Ben Burr, Brookhaven National Laboratory
Tim Helentjaris, University of Arizona
Scott Tingey, DuPont Experimental Station

The course is designed to explore both theoretical and practical concepts
on the use of molecular markers in plant genetics and plant breeding.
Participants will learn a variety of techniques that can be used to
approach problems 


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