DNA COURSE OFFERING

Jane Clay, Continuing Studies, 5-6329 JCLAY at ucs.indiana.edu
Wed Jan 25 19:13:10 EST 1995


The following message contains information about two DNA course 
offerings at Indiana University, including a registration form that may 
be printed, completed, and returned.



Recombinant DNA:  Techniques and Application

This summer, Indiana University will offer two laboratory-based short
courses that provide the opportunity to learn and apply recombinant 
DNA procedures and the technologies of sequencing, PCR analysis, and 
RFLP analysis. Courses dealing with the technology of DNA analysis are 
in their thirteenth year at Indiana University, and they have received 
high praise for excellent instruction and immediate applicability.

The application of this technology is relatively easy to understand and 
is comparatively economical in time, equipment, and materials. 
Participants in these courses will learn to perform these techniques 
and may arrange to work on a sample of genomic DNA from their own 
research organism.  Participants are expected to include

* college or high school educators who will learn to perform and teach 
these techniques.
* industrial or other laboratory researchers considering the 
application of recombinant DNA technology.
* others interested in the application of this technology in society.



Special features of these courses

* Participants will have the opportunity during the courses to isolate 
and work with genomic DNA from their own research organism. Call 
812-855-6329 to make the necessary arrangements.
* Participants will receive a registration discount when they enroll in 
both courses.

In these courses, learn techniques and applications that will allow you 
to
* advance your own research.
* isolate and/or sequence DNA that you bring.
* obtain valuable experience for teaching DNA technology in the  
classroom.
* gain familiarity with the organization of the human genome.



I. Recombinant DNA Technology
June 4-9, 1995

Participants in this course will learn the following techniques:
* DNA and cloning vector manipulation
* PCR technology
* Preparation of recombinant DNA
* Transformation of bacterial cells
* Selection and assay of cloned and amplified fragments of "foreign" 
DNA
* Transfer of DNA for probing (Southern blot)
* Preparation of nonradioactive DNA probes

This course is designed for those with a basic understanding of the
structure of DNA and with a minimal understanding of enzymes and
biochemistry. Most of the procedures taught to biology graduate 
students in the recombinant DNA section of a graduate techniques 
course at Indiana University will be covered.



II. Application of Recombinant DNA Technology: RFLP and 
Fingerprinting Analysis, RAPD Analysis, and DNA Sequencing
June 11-16, 1995

The following techniques will be emphasized:
* DNA sequencing
* RAPD analysis of genomic DNA
* Fingerprinting and RFLP analysis of genomic DNA
* Electroporation of bacterial cells
* Chemiluminescent detection of nucleic acids
* Application of computers to DNA sequencing data analysis
* Preparation of random fragment sequencing libraries and double 
stranded DNA for sequencing
* Use of BioNeb cell and biopolymer disruption systems

This course is designed for those with a basic understanding of the
structure of DNA and with a minimal understanding of enzymes and
biochemistry. Previous experience with PCR analysis, RFLP analysis, 
and DNA sequencing is not a prerequisite, nor is completion of the 
Recombinant DNA Technology course.



Faculty

The instructor for these courses will be Dr. Stefan J. Surzycki, 
associate professor of biology at Indiana University. Dr. Surzycki 
teaches laboratory classes employing the techniques introduced in 
these courses. His research focuses on development of large-scale DNA 
sequencing techniques, computer analysis, and genomic screening. Dr. 
Surzycki is also co-inventor of the BioNeb cell disruption system, a 
device that gently breaks cells and randomly shears DNA into uniform 
fragments of chosen size. The BioNeb was named one of the 100 most 
technologically significant new products of 1993.

Dr. Surzycki will be assisted in each course by Judith Surzycki, a 
teaching associate in biology with extensive experience teaching the 
techniques of these courses, and by an advanced doctoral student in 
biology.



Schedule

Each short course will open with a Sunday evening reception. In 
addition to the daytime laboratory schedule, lectures and continued 
lab work are planned for three evenings.



Location

Bloomington, home of Indiana University, is a town of 60,000 located 
50 miles southwest of Indianapolis. Bloomington offers the hospitality 
of a small town with the entertainment, shops, and restaurants of a 
city.  Indiana University's wooded campus is surrounded by hills and 
lakes that provide many recreational opportunities.



Housing

Hotel accommodations and campus dormitory housing are available for 
each program, and room rates are moderate. Detailed information 
about travel routes to Bloomington, accommodations, meals, and 
parking will be sent to participants as soon as registrations are 
received.



Tuition

The fee for each course is US$925. Participants in both courses pay a 
total discounted fee of US$1,500. The fee includes all instruction, 
laboratory supplies, use of equipment, and an extensive lab manual in 
each class. An opening reception and coffee breaks are also included. 
Housing, parking, and meals are not included.



Registration

Registration deadline is May 19, 1995, for both courses. Enrollment is 
limited.

Continuing Education Units

Four continuing education units (CEUs) will be earned for completion 
of each program. CEUs are nationally recognized units earned through
participation in certified noncredit continuing education programs.



Registration Form (to be printed, filled out, and returned)
(deadline May 19, 1995)
Please check appropriate box(es).

/ / I. Recombinant DNA Technology (June 4-9)

/ /  II. Application of Recombinant DNA Technology: RFLP and 
Fingerprinting Analysis, RAPD Analysis, and DNA Sequencing 
(June 11-16)

Name _____________________________________

Position ___________________________________

Organization
or School __________________________________

Address ___________________________________

City ______________________________________

State ____________  Zip _____________________

Work Phone ________________________________

Home Phone _______________________________


Fee for one program: US$925
Discounted fee for both programs: US$1,500
Amount enclosed: US$________________
Make check or money order payable to Indiana University. All or part 
of the cost of this program may be tax deductible. For further 
information, contact your local IRS office or your tax consultant.

/ / VISA   / / MasterCard  / / Discover

Credit Card Number ___________________________
Expiration Date ______________________________
Name on Card (Please print)
__________________________________________
Signature of Card Holder
__________________________________________

Mail your registration and check (made payable to Indiana University) 
to:  Division of Continuing Studies, Owen Hall 204, Indiana University,
Bloomington, IN 47405 or fax your registration (with completed 
credit card information) to Jane Clay at 812-855-8997

For additional information contact:
Jane Clay, Division of Continuing Studies
Phone: 812-855-6329    Fax: 812-855-8997
Internet: JClay at Indiana.EDU
or
Dr. Stefan Surzycki, Department of Biology
Phone: 812-855-4880



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