One Day Symposium on Gene Expression and Mutation Analysis

[Li Jin]

Gene Expression and Mutation Analysis

Holiday Inn, Foster City, CA 
April 16th, 1996 (Tuesday)
[Sponsored by the Californian Separation Science Society]

 8:45 -  9:00 am  Gene expression and mutation analysis - a perspective.
                  To be named

Quantitation of Gene Expression

 9:00 -  9:30 am  Development of branched DNA (bDNA) signal amplification    
                  assays for the direct quantitation of gene expression.
                    Janice A. Kolberg 
                    Nucleic Acid Systems, Chiron Corp., Emeryville, CA
 9:30 - 10:00 am  Accurate quantitation of gene expression by single-tube 
                  competitive RT-PCR and HPLC.
                    Peter A. Doris 
                    Department of Cell Biology and Biochemistry, Texas  
Tech 
                    University, Lubbock, TX
10:00 - 10:30 am  Characterization of gene expression in single neurons by 
                  patch-clamp and single-cell RT-PCR.
                    Lillian W. Chiang 
                    Department of Neurobiology, Stanford University, CA

10:30 - 11:00 am  Coffee Break

Gene Expression and Function Analysis

11:00 - 11:30 am  Parallel gene expression monitoring on high-density cDNA 
                  microarrays.
                    Mark Schena
                    Department of Biochemistry, Stanford University, CA
11:30 - 12:00 am  High-throughput functional analysis of open reading  
                  frames using tagged deletion strains and high-density 
                  oligonucleotide arrays.
                    Dan Shoemaker
                    Department of Biochemistry, Stanford University, CA
12:00 - 12:30 am  Genomic Analysis of Gene Function in Yeast using Genetic 
                  Footprinting.
                    Victoria Smith
                    Department of Biochemistry, Stanford University, CA 

12:30 -  1:30 pm  Lunch

In Search of Unknown Mutations

 1:30 -  2:00 pm  Hybrid SSCP methods for detecting essentially 100% of      
                  mutations
                    Hiroshi Nishino
                    Department of Biochemistry and Molecular Biology, Mayo 
                    Medical School, Rochester, MN.
 2:00 -  2:30 pm  Enzymatic methods for mutation detection.
                    Christopher D. Earl
                    Avitech Diagnostics, Malvern, PA
 2:30 -  3:00 pm  Development and application of an in vivo method to    
                  detect DNA sequence variation.
                    Malek Faham
                    Department of Genetics, Stanford University, CA
 3:00 -  3:30 pm  High-performance liquid chromatography of nucleic acids      
                  and its application to comparative DNA sequencing.
                    Peter Oefner
                    Department of Biochemistry, Stanford University, CA

 3:30 -  4:00 pm  Coffee Break

>From Sequence to Genotype

 4:00 -  4:20 pm  Direct sequencing of PCR products. 
                    Peter Underhill
                    Department of Genetics, Stanford University, CA
 4:20 -  4:40 pm  Heterozygote sequencing of HLA-polymorphisms
                    Mel Kronick
                    Perkin-Elmer, Applied Biosystems Division, 
                    Foster City, CA
 4:40 -  5:10 pm  Closed-tube PCR analysis with fluorogenic probes for 
                  very high throughput sequence detection and  
                  quantitation.
                    Kenneth Livak
                    Perkin-Elmer, Applied Biosystems Division, 
                    Foster City, CA
 5:10 -  5:40 pm  PCR-OLA-SCS for massive-multiplex genetic testing.
                    William Bloch
                    Perkin-Elmer, Applied Biosystems Division, 
                    Foster City, CA 
 5:40 -  6:10 pm  Generation of bi-allelic markers and their use in 
                  automated gene localization. 
                    Li Jin
                    Department of Genetics, Stanford University, CA

 6:10 -  8:00 pm  Microbrewery Debriefing and Tasting

For further information, please contact 
    Dr. Peter Oefner 
    at (415) 725-6117, oefner at genome.stanford.edu or 
    Ms. Joan Salucci
    at Californian Separation Science Society at (415) 512-1032,  
society at hooked.net.

Registration information: 
   Advance registration (before April 1, 1996): 
      CaSSS members       $100.00
      non-CaSSS members   $125.00
      students            $ 50.00
   Late registration (after April 1, 1996):
      CaSSS members       $125.00
      non-CaSSS members   $175.00
      students            $ 75.00
(Registration fee includes admission, hand-out, lunch, and microbrewery  
tasting.)