In Vitro Toxicology Workshop at ATCC

[Mr. Ken Carter]

*********IN VITRO TOXICOLOGY: TECHNIQUES AND APPLICATIONS WORKSHOP********
		American Type Culture Collection (ATCC)
			JUNE 4-7, 1996
		Rockville, MD   workshops at atcc.org
		http://www.atcc.org/workshops/workshop.html	

**(2 all expense-paid scholarships to this workshop are available to 
post-docs.  Please e-mail me for more information).

This four-day laboratory-intensive course proceeds in a logical manner 
from basic concepts of tissue culture and toxicity testing to specialized 
systems for evaluating organ-specific toxicity. There will be a special 
emphasis on in vitro ocular and dermal toxicity tests which have been the 
focus of much recent validation activity and are now widely used for 
topical toxicity testing. Participants will begin by setting up cultures 
of a simple cell line, exposing cells to graded concentrations of a toxic 
agent and performing basic cytotoxicity assays. A variety of different 
cultured cell types will be available for examination. Participants will 
then learn a variety of the most successful alternatives to the Draize 
eye and skin tests. Ocular toxicity tests will include the hen's egg test 
(HET-CAM) and the bovine corneal opacity test (BCOP). Dermal toxicity 
tests will utilize trans filter cultures of human skin constructs. The 
workshop will be completed by preparing and culturing liver slices using 
the precision-cut slice technique. These will be used in a study of 
xenobiotic-induced toxicity.

The broad hands-on experience and detailed protocols provided by this 
workshop should give attendees the necessary confidence and information 
to monitor or conduct these studies in their own laboratories. In 
addition, there will be time for informal discussions of issues of 
interest to individual participants. This workshop would most benefit 
those with an immediate or foreseeable need to use in vitro methods to 
address questions of toxicity or risk assessment. A familiarity with 
tissue culture techniques would be useful, but is not required for 
attendance.

Faculty: Dr. Oliver Flint (Workshop Director), Bristol-Myers/Squibb; Dr. 
Joseph Sina, Merck Research Laboratories; Dr. Carol Greene, SRI 
International; and Mr. Charles Noble, MSDRL-Safety Assessment, Dr. John 
Harbell, Microbiological Associates.

Limited to 24 participants
FEE: $875.00
2.8 CEUs 
Please contact workshops at atcc.org for registration information and for a 
copy of the full schedule.

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Ken Carter			           ^	
Department of Biological Sciences        / l \       (410) 455-2271
Univ. of Maryland Baltimore County     /___l___\__   fax:(410) 455-3875
Baltimore, Maryland  21228   	       \______/      kcarter at umbc.edu
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