Freezing & Freeze-Drying Workshop

Mr. Ken Carter kcarter at
Tue Oct 7 22:59:59 EST 1997

				October 27-30, 1997
		American Type Culture Collection (ATCC), Rockville, MD

This four-day course is designed for scientists and technical personnel in
the fields of Bacteriology, Mycology, Protistology and Virology who need
reliable methods of culture preservation. It has been expanded to four
days of lecture, discussion, demonstration and hands-on laboratory

Lecture topics include cellular responses to ice crystal formation,
changes in isotonicity of the cellular environment, rates of cooling,
latent heat of fusion, sublimation dynamics, collapse, melt-back factors
affecting the viability of frozen and freeze-dried microorganisms,
importance of residual moisture and methods for determining residual
moisture, cryoprotectants and other additives, and shelf life prediction.
The benefits and drawbacks of each method are discussed, as well as
optimum storage rehydration and thawing conditions.

Laboratory sessions include several ATCC methods for freezing and
freeze-drying; use of bench top commercial sublimators, preparation of
cells, cryopreservation and lyophilization; torch sealing, rehydration and

A copy of the "ATCC Preservation Methods Manual" will be included with the
course material.

FACULTY: M. T. Alexander, B.S. (Workshop Director), Frank P. Simione, Jr.,
ATCC Director, Professional Services; Ronald L. Jewett, ATCC Operations;
Michael J. Pikal, Ph.D. (invited), Sr. Research Scientist, Lilly Research
Laboratories; Willaim F. Rall, Ph.D., Cryobiologist, National Institutes
of Health.

Limited to 24 participants
FEE: $1,195.00
2.8 CEUs

**For a full schedule and on-line registration, please visit our Web site

**Or request a brochure, which includes a full schedule and registration
form from: kcarter at
or call 1-800-359-7370

Ken Carter			          ^	
American Type Culture Collection        / | \       (301) 231-5525
12301 Parklawn Drive                  /___|___\__   fax:(301) 816-4364
Rockville, Maryland  20852             \______/      kcarter at

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