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ADVANCED Recombinant DNA Workshop

Mr. Ken Carter kcarter at umbc.edu
Wed Jul 8 09:29:50 EST 1998

AUGUST 17-21, 1998
American Type Culture Collection (ATCC)
Location: This workshop will be held at the PDA Training and Research
Institute in Catonsville, Maryland

workshops at atcc.org

This five-day laboratory-intensive workshop will provide intermediate and
advanced instruction in cDNA cloning, detection, sequencing, and
manipulation. Hands-on laboratory experience will be supplemented with
lectures and discussions. Participants should be familiar with the
techniques and concepts presented in the ATCC Recombinant DNA:  Techniques
and Applications workshop, such as molecular cloning, enzymes, vectors, E.
coli host genotypes library construction, nucleic acid sequencing,
restriction mapping, probes and PCR, and synthesis and cloning of cDNA.

This workshop will focus specifically on the detection and measurement of
mRNA  expression, cDNA analysis, and biosynthetic/transgenic techniques.
The workshop will consist of hands-on exercises built around actual
laboratory protocols. These exercises are supported by lectures pertaining
to each of the techniques, as well as problem solving, and reagent
preparation. We have chosen some of the most commonly employed techniques,
such as Northern blotting, magnetic bead mRNA isolation, RTPCR, cDNA
library construction, lipofection, and electroporation. These techniques
can complement existing molecular biology research or can form the
foundation for a new foray into this field.

For research material, we will use mouse embryonic stem (ES) cells,
embryonic germ (EG) cells, and tissues. ES and EG cells are of particular
interest, as their pluripotentcy allows for genetic manipulation of the
mouse germ line, leading to gene knockOut and transgenic mice.

Students are encouraged to thoroughly read the pertinent lectures and
laboratory exercises prior to their presentation. Lecture and exercise
schedules are provided in the workshop manual. In addition to the manual,
lectures, and exercises, we will work with the students to formulate
questions and choose methods that are applicable to their own research

Faculty: Dr. Michael J. Shamblott, Workshop Director, Johns Hopkins
University School of Medicine; Dr. Suria Pancharatnam, JHU; Beth Bugg,
JHU; Deborah Howard, JHU; Scott Durkin, ATCC.

Limited to 24 participants
FEE: $1,395.00
3.5 CEUs

Ken Carter			          ^	
American Type Culture Collection        / | \       (703) 365-2726
10801 University Blvd.                /___|___\__   fax:(703) 365-2770
Manassas, Virginia  20110              \______/      kcarter at atcc.org

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