Cold Spring Harbor Laboratory Courses - Deadline March 15th

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Thu Mar 9 11:40:56 EST 2000

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MARCH 15th Deadline
Cold Spring Harbor Course
Advanced Bacterial Genetics
June 7 - 27, 2000
Bonnie Bassler, Princeton University
Colin Manoil, University of Washington, Seattle
James Slauch, University of Illinois, Champagne-Urbana
The laboratory course will present logic and methods used in the genetic 
dissection of complex biological processes in bacteria. The methods to
presented include: mutagenesis using transposons, mutator strains, and 
chemical and physical mutagens; mapping mutations using genetic and 
physical techniques; generation and analysis of gene fusions; molecular 
cloning; Polymerase Chain Reaction; Southern blot analysis; epitope 
insertion mutagenesis; and site-directed mutagenesis. A key component of 
the course will be the use of sophisticated genetic methods in the
of pathogenic and "undomesticated" bacteria. Invited lecturers will 
describe the use of genetic approaches to study biological processes in
variety of bacteria.

Cold Spring Harbor Course
Genetic-Epidemiological Studies of Complex Diseases
June 7 - 13, 2000
Neil Risch, Stanford University
Elizabeth Squires-Wheeler, Columbia University College of Physicians & 
This lecture course will consider the difficulties in studying the
basis of complex disorders, such as diabetes, cardiovascular disease, 
cancer, Alzheimer's disease, schizophrenia and epilepsy, with a
emphasis on neuropsychiatric conditions. We will discuss 
genetic-epidemiologic study designs, including family, twin and adoption 
studies, as well as mode of inheritance analyses, and their role in
the framework for understanding the genetic and non-genetic components
of a 
disease. A major focus will be the identification of specific gene
using both linkage and association analysis. We will discuss the
and robustness of different designs for such analysis; further we will 
consider how evidence from genetic-epidemiologic studies informs both
design and interpretation of molecular genetic studies. Recent
of genes for both Mendelian and non-Mendelian diseases will guide the 
discussion of the various methodologic issues.   We look forward to
you at Cold Spring Harbor. 	

Cold Spring Harbor Course
Molecular Embryology of the Mouse
June 7 - 27, 2000
Andras Nagy, Samuel Lunenfeld Research Institute, Canada
Patrick Tam, Children's Medical Research Institute, Australia
This intensive laboratory/lecture course is designed for biologists 
interested in applying their expertise to the study of mouse embryonic 
development. Laboratory components provide an introduction into the 
technical aspects of working with and analyzing mouse embryos, and
provide the conceptual basis for contemporary research in mouse 
development. Experimental techniques that will be covered in the
include in vitro culture and manipulation of pre- and post-implantation 
embryos, transgenesis by DNA microinjection, embryo transfer, establ 
ishment, culture and genetic manipulation of embryonic stem cells, 
production of chimaeras by aggregation with and injection of embryonic
cells and the analysis of development by whole mount in situ
skeletal preparation and transgene expression. Last year's speakers
Siew-Lan Ang, Richard Behringer, Benoit de Crombrugghe, Achim Gossler, 
Richard Harvey, Brigid Hogan, Nancy Jenkins, Alexandra Joyner, Peter 
Koopman, Robin Lovell-Badge, Nobuja Maeda, Terry Magnuson, Anne McLaren, 
Andy McMahon, Gail Martin, Andras Nagy, Virginia Papaioannou, Oliver 
Smithies, Davor Solter and Patrick Tam.
Cold Spring Harbor Course
Physiological Approaches to Ion Channel Biology
June 7 - 27, 2000
Angeles B. Ribera, University of Colorado Health Sciences Center
Laurence O. Trussell, Vollum Institute
Thomas S. Otis, University of California, Los Angeles
This intensive laboratory/lecture course will introduce students to 
electrophysiological approaches for the study of ion channels in their 
native environments. Hands-on exercises will include patch clamp
of ion channel activity in acutely isolated or cultured cells or neurons
brain slice preparations. Different recording configurations (e.g., 
whole-cell, nucleated-patch, perforated whole-cell, single channel) will
used. The advantages and disadvantages of each method and preparation
be considered in order to match experimental approaches to scientific 
questions. Similarly, various methods for ligand/drug application will
demonstrated. A primary goal will be to identify properties of ion
that allow neurons and non-neuronal cells to carry out their unique 
physiological or systems-level roles. These properties will be related
information previously gained from molecular cloning and heterologous 
expression of ion channel genes. Areas of particular interest include 
channels that (1) are activated by neurotransmitter at central and 
peripheral synapses, (2) interact with other channels to shape the
properties of neurons, (3) respond to neuromodulators with changes in 
functional properties, or (4) are developmentally required and
The research interests of guest lecturers will complement and expand
list. This course is intended for students with specific plans to apply 
these techniques to a defined problem, and students are encouraged to
their preparation to the course for preliminary studies
Cold Spring Harbor Course
Computational Neuroscience: Vision
June 15 -28
Eero Simoncelli, New York University
Eduardo-Jose Chichilinsky, The Salk Institute
Paul W. Glimcher, New York University
Computational approaches to neuroscience have produced important
in our understanding of neural processing. Prominent successes have come
areas where strong inputs from neurobiological, behavioral and 
computational approaches can interact. Through a combination of lectures 
and hands-on experience in a computer laboratory, this intensive course 
will examine areas, including color vision, spatial pattern analysis, 
binocular stereopsis, motion analysis, oculomotor function, attention,
object representation. The theme is that an understanding of the 
computational problems, the constraints on solutions to these problems,
the range of possible solutions can help guide research in neuroscience. 
Students should have experience in neurobiological or computational 
approaches to visual processing. Some background in mathematics, and 
familiarity with computers will be beneficial. Past lecturers have 
included: Edward Adelson, Richard Andersen, David Brainard, Heinrich 
Bulthoff, Denis Dacey, Robert Desimone, Rudiger von der Heydt, Norma 
Graham, Ellen Hildreth, Peter Lennie, Stephen Lisberger, Jitendra Malik, 
John Maunsell, Suzanne McKee, Michael Morgan, Ken Nakayama, Izumi
William Newsome, John Palmer, Tomaso Poggio, Jeff Schall, Terrence 
Sejnowski, David Sparks, Keiji Tanaka, Shimon Ullman, and Brian Wandell.	

Cold Spring Harbor Course
Making and Using DNA Microarrays
June 21 - 27
Joseph DeRisi, University of California, San Francisco
Vishwanath Iyer, Stanford University Medical Center
A DNA microarray is a simple, inexpensive and versatile tool for 
experimental explorations of genome structure, gene expression programs, 
gene function, and cell and organismal biology. In this hands-on course, 
students will be guided through the process of preparing DNA samples and 
slides to be used for printing microarrays, printing DNA microarrays, 
designing and conducting experiments for analysis by DNA microarray 
hybridization, data analysis, display and interpretation. Experimental 
applications to be covered in the course will include systematic studies
global gene expression programs, inferring gene function using
genotyping, and measuring changes in gene copy number. Students who 
complete this course should be fully able to set up their own
facility for printing and experimental use of DNA microarrays. Guest 
instructors will present the state-of-the-art in new technology, 
experimental applications, and interpretation of large genomic datasets.
Cold Spring Harbor Course
Arabidopsis Molecular Genetics
June 30 - July 20
Kathy Barton, University of Wisconsin, Madison
John Bowman, University of California, Davis
Ueli Grossniklaus, Friedrich Miescher Institute, Switzerland
This course provides an intensive overview of topics in plant growth, 
physiology and development, focusing on molecular genetic approaches to 
understanding plant biology. It emphasizes recent results from
thaliana and other model plants and provides an introduction to current 
methods used in Arabidopsis research. It is designed for scientists with 
experience in molecular techniques or in plant biology who wish to work 
with Arabidopsis. The course consists of a vigorous lecture series, a 
hands-on laboratory, and informal discussions. Discussions of important 
topics in plant research will be presented by the instructors and by 
invited speakers. These seminars will include plant morphology and
plant development (including development of flowers, roots, meristems
leaves, male and female gametophytes, and embryos); perception of light
photomorphogenesis; synthesis, function and perception of hormones. 
Lectures describing bioinformatics tools available to the Arabidopsis 
community, and the potential of the Arabidopsis genome project for 
accelerating Arabidopsis research will also be included. Speakers will 
provide overviews of their fields, followed by in-depth discussions of 
their own work. The laboratory sessions will provide an introduction to 
important techniques currently used in Arabidopsis research. These
studies of Arabidopsis development, mutant analysis, in situ detection
RNA, histochemical staining, transient gene expression, applications of 
green fluorescent protein fusions, protein interaction and detection, 
techniques commonly used in genetic and physical mapping, map-based 
cloning, and mutant isolation by reverse genetics. The course also
several short workshops on important themes in genetics.
Last year's speakers included: Philip Benfey, Winslow Briggs, Zach
Joanne Chory, Jerry Cohen, Xing-Wang Deng, Nancy Dengler, Thomas Jack, 
David Jackson, Nancy Kerk, Rob Martienssen, Peter McCourt, Ann
Scott Poething, Eric Richards, David Spector, Ian Sussex, Michael
Athanasios Theologis, Albrecht Von Arnim, Susan Wessler, & Detlef
We look forward to seeing you at Cold Spring Harbor.
Cold Spring Harbor Course
Molecular Cloning of Neural Genes
June 30 - July 20, 2000
James Boulter, University of California, Los Angeles
Catherine Dulac, Harvard University
Cary Lai, The Scripps Research Institute
Daniel Lavery, Glaxo Wellcome

This intensive laboratory and lecture course is intended to teach 
neuroscientists current approaches to molecular neurobiology. The course 
consist of daily lectures and laboratory exercises on the practice of 
molecular neurobiology, with an emphasis on modern approaches to cloning 
and analyzing the expression of neural genes. A series of evening
seminars by invited speakers focuses on the ways in which these
techniques have been successfully applied. In the past, evening seminar 
topics have included expression cloning, single cell cloning,
cDNA cloning strategies, and genetic and mechanistic studies of
disease, aquisition of cell identity and axon guidance in the developing 
nervous system. The laboratory portion of the course begins with 
instruction in a series of basic molecular biological techniques and 
rapidly advances to more sophisticated methodologies. Students learn to 
prepare genomic, phage and plasmid DNAs, total and poly (A)+ RNA, and to 
generate and screen cDNA libraries. Additional topics and methods
include restriction mapping, agarose and polyacrylamide gel 
electrophoresis, Northern and Southern blotting, subcloning, 
oligonucleotide primer design, a selection of PCR-based techniques and
use of nucleotide and protein sequence databases. Gene expression
include the production of fusion proteins in bacteria and mammalian cell 
transfection. The advanced techniques feature the construction of cDNA 
libraries from single cells and the use of subtractive cDNA methods to 
clone genes expressed in limited populations of cells.
Neurobiology of Drosophila
June 30 - July 20
Kendal Broadie, University of Utah
Guy Tear, King's College London
Steven de Belle, University of Nevada
This laboratory/lecture course is intended for researchers at all levels 
from beginning graduate students through established primary
who want to use Drosophila as an experimental system for nervous system 
investigation. The three week course is divided into the study of 
development, physiology/function and behavior. Daily seminars
introduce students to a variety of research topics and develop those
by including recent experimental contributions and outstanding questions
the field. Guest lecturers bring original preparations for viewing and 
discussion and direct laboratory exercises and experiments in their area
interest. The course provides students with hands-on experience using a 
variety of experimental preparations that are used in the investigation
current neurobiological questions. The lectures and laboratories will
on both the development of the nervous system and its role in
larval and adult behaviors. In addition to an exposure to the molecular 
genetic approaches available in Drosophila, students will learn a
of techniques including: embryo in situ hybridization, labelling of 
identified neurons, electrophysiological recording from nerves and
and the analysis of larval and adult behavior. Collectively, the course 
will provide a comprehensive and practical introduction to modern 
experimental methods for studying the Drosophila nervous system. The 
specific topics from last year's course included: neurogenesis, axon 
pathfinding, synaptogenesis, membrane excitability, synaptic function
plasticity, photoreception, mechanoreception, neural circuits,
rhythms, courtship, learning and memory, locomotory and flight
Last year's lecturers included: Andrea Brand, Volker Hartenstein, Kei
Maurice Kernan, Rod Murphey, Kevin O'Dell, Andreas Prokop,
Marla Sokolowski, Roland Strauss, Jessica Triesman, Tim Tully, Yi Zhong, 
Konrad Zinsmaier.
Cold Spring Harbor Course
Mouse Behavioral Analysis
July 7 - 20

Michael Fanselow, University of California, Los Angeles
Michela Gallagher, Johns Hopkins University, Baltimore
Mark Mayford, University of California, San Diego
Alcino Silva, UCLA Medical Center
Dear Colleague:

This course is intended to provide a theoretical and experimental 
introduction to behavioral analysis in the mouse, with a focus on
and memory. It is specially designed for geneticists, molecular
pharmacologists, and electrophysiologists with a need for a hands-on 
introduction to behavioral analysis of the mouse. Additionally, the
will cover the principles of using mutant mice in behavioral studies, as 
well as the issues involved in integrating behavioral, neuroanatomical, 
neurophysiological, and molecular findings. Among the methods presented 
will be the Water Maze, Cued and Contextual Fear Conditioning, 
Natural/Ethologically-Relevant Learning, Open Field Behavior, the
and other Activity Tests. In addition, there will be demonstrations of 
several aspects of in vitro electrophysiology (fields and whole-cell 
recordings of synaptic plasticity).
We look forward to seeing you at Cold Spring Harbor.
Cold Spring Harbor Course
Brain Development & Function
July 6 - 19
Ronald McKay, National Institutes of Health
Michael Posner, Weill Medical College of Cornell University
This is an advanced lecture and discussion course on the development and 
function of the nervous system. 20 participants with diverse
and intellectual backgrounds meet for two weeks with leading
researchers. The lectures provide both a comprehensive introduction as
as a detailed present of current research. There are many opportunities
interaction. This is an unusual opportunity to review contemporary ideas 
about the brain with leading scientists. In our last meeting, the
included Ann-marie Craig, Howard Eichenbaum, Marla Feller, David Ginty, 
Kirsten Harris, Rick Huganir, Alex Kolodkin, Jeff Lichtman, Jennifer 
Lippincott-Schwarz, Giles Laurent, Tim Mitchison, Bob Muller, Lou 
Reichardt, Tim Ryan, Erin Schuman, Tito Serafini, Rachel Wong, and
Yuste. The specific topics covered will include neurogenetics, stem cell 
differentiation, cell and gene therapy, axon guidance, synapse
synaptic plasticity, hippocampal function, neuroimaging methods, human 
brain development and aging. The goal is to provide a critical view of
different levels of knowledge that are required for an integrated 
understanding of the brain.	
Cold Spring Harbor Course
July 21 - 27, 2000
How and why do neurons die in specific acute or chronic human
disorders? What are the molecular and biochemical manifestations of 
specific genetic lesions in specific neurodegenerative disorders? Do 
different pathological deaths share common mechanisms? What practical 
treatments can be contemplated? This lecture course will explore
answers to these important questions. Recent advances in neurogenetics
in molecular and cell biology have begun to shed light on the mechanisms 
that underly nervous system injury in disease states such as Alzheimer's 
disease, Parkinson's disease, frontotemporal dementia, amyotrophic
sclerosis, prion diseases, and polyglutamine repeat disorders. Taking 
advantage of small class size and extensive discussion, invited faculty 
lecturers will examine critical issues in their areas of expertise. 
Overview will be provided and course participants need not have
with neurological diseases. The course will focus principally on the 
specific hypotheses and approaches driving current research. Emphasis
be placed on the highly dynamic interface between basic and clinical 
investigation, including the interdependence of clinical research and 
disease model development, and the value of disease research in 
understanding the function of the normal nervous system. Last year's 
lecturers were: John Hardy, Don Cleveland, Marcy MacDonald, Danilo
Harry Orr, Adriano Aguzzi, Rudi Glockshuber, Virginia Lee, John 
Trojanowski, John Morris, David Holtzman, Ed Koo, Sam Gandy, Jorge
Dennis Selkoe, Rudy Tanzi and David Borchelt.	
Cold Spring Harbor Course
C. elegans
July 25 - August 14
Michael Hengartner, Cold Spring Harbor Laboratory
Erik Jorgensen, University of Utah
Ronald Plasterk, Netherlands Cancer Institute
This course is designed to familiarize investigators with C. elegans as
experimental system, with an emphasis on both classical genetic analysis 
and reverse genetic approaches. A major goal is to teach students how to 
successfully exploit the information generated by the C. elegans genome 
project. The course is suited both for those who have a current training
molecular biology and some knowledge of genetics, but have no experience 
with C. elegans, as well as students with some prior worm experience who 
wished to expand their repertoire of expertise. The following topics
be covered both in the laboratory and by lectures from experts in the 
field: worm pushing, C. elegans databases and worm bioinformatics,
and development, forward genetics, chemical and transposon mutagenesis, 
generation of transgenic animals, expression pattern analysis, reverse 
genetics, construction and screening of deletion libraries, and RNA 
inactivation. The course is designed to impart sufficient training to 
students in the most important attributes of the C. elegans system to 
enable students to embark on their own research projects after returning
their home institutions.
We are looking forward to a most informative and exciting meeting. We
forward to seeing you at Cold Spring Harbor.
Cold Spring Harbor Course
Eukaryotic Gene Expression
July 25 - August 14
Brian Dynlacht, Harvard University
Grace Gill, Harvard Medical School
James Goodrich, University of Colorado
Jacqueline Lees, Massachusetts Institute of Technology
Dear Colleague:

The Eukaryotic Gene Expression Course is designed for students,
and principal investigators who have recently ventured into the dynamic 
area of gene regulation. The course will focus on state-of-the-art 
strategies and techniques employed in the field. Cell-free extracts for
vitro transcription will be prepared and RNA levels measured by primer 
extension analysis. An emphasis will be placed on biochemical studies of 
protein-DNA and protein-protein interactions. A detailed
of the DNA-binding properties of a site-specific transcription factor
be carried out using electrophoretic mobility shift and DNase I foot 
printing assays. These assays will be used to study protein-DNA 
interactions in crude extracts and using recombinant proteins purified
class. Both affinity chromatography and co-immunoprecipitation methods
be employed to investigate protein-protein interactions with the general 
transcription machinery. Over the past few years, the gene regulation
has begun to emphasize the importance of in vivo approaches to studying 
protein-DNA and protein-protein interactions. Students will therefore be 
exposed to in vivo footprinting, mapping of DNAse 1 hypersensitive
and the yeast two-hybrid methodologies. Experience with basic
DNA techniques is a prerequisite for admission to this course. Lectures
the instructors will cover the current status of the gene expression
theoretical aspects of the methodology, and broader issues regarding 
strategies for investigating the regulation of gene expression in 
eukaryotes. Guest lecturers will discuss contemporary problems in 
eukaryotic gene regulation and technical approaches to their solution.
speakers last year included T. Archer, D. Bentley, K. Blackwell, S.
M. Carey, L. Freedman, N. Hernandez, D. Price, J. Reese, S. Smale, J. 
Workman, R. Young and K. Zaret.
We are looking forward to a most informative and exciting meeting. We
forward to seeing you at Cold Spring Harbor.
Cold Spring Harbor Course
Imaging Structure and Function in the Nervous System
July 25 - August 14
Kerry Delaney, Simon Fraser Laboratory
Venkatesh Murthy, Harvard University
Karel Svoboda, Cold Spring Harbor Laboratory
Dear Colleague:
Advances in light microscopy, digital image processing, and the
of a variety of powerful fluorescent probes present expanding
for investigating the nervous system, from synaptic spines to networks
the brain. This intensive laboratory and lecture course will provide 
participants with the theoretical and practical knowledge to utilize 
emerging imaging technologies. The primary emphasis of the course will
on vital light microscopy. Students will learn the principles of light 
microscopy, as well as use of different types of electronic cameras,
scanning systems, functional fluorophores, delivery techniques, and
image-processing software. In addition to transmitted light microscopy
viewing cellular motility, the course will examine a variety of
probes of cell function, including calcium-sensitive dyes, 
voltage-sensitive dyes, photo-activated ("caged") compounds, and
tracers. Issues arising in the combination of imaging with 
electrophysiological methods will be covered. Particular weight will be 
given to multi-photon laser scanning microscopy and to newly available 
biological fluorophores, especially Green-Fluorescent Protein (GFP) and
variants. We will use a spectrum of neural and cell biological systems, 
including living animals, brain slices, and cultured cells. Applicants 
should have a strong background in the neurosciences or in cell biology. 
Lecturers in the 1999 course included: Chiye Aoki, William Betz, Tobias 
Bonhoeffer, Andrea Brand, John Connor, Richard Day, Paul Forscher,
Helmchen, Daniel Jay, Rex Kerr, David Kleinfeld, Jeff Lichtman, Greg 
Macquire, Andrew Matus, Christina Rapp, Williams Schafer, Karel Svoboda, 
Lansing D. Taylor, Roger Tsien, Clare Waterman-Store, and Shimon Weiss.
We look forward to seeing you at Cold Spring Harbor.
Cold Spring Harbor Course
Yeast Genetics
July 25 - August 14
Daniel Burke, University of Virginia
Orna Cohen-Fix, NIDDK, National Institutes of Health
Dean Dawson, Tufts University School of Medicine
Tim Stearns, Stanford University
The Yeast Genetics Course is a modern, state of the art laboratory
designed to teach the students the full repertoire of genetic approaches 
needed to dissect complex problems in the yeast Saccharomyces
Combinations of classical genetic approaches are emphasized, including
isolation and characterization of mutants, tetrad analysis, 
complementation, and mitotic recombination. Molecular genetic
including various types of yeast transformation, gene replacement with 
plasmids and PCR, construction and analysis of gene fusions, and
of mutations in cloned genes, will also be emphasized. Students will use 
the classical and molecular approaches to gain experience in identifying 
and interpreting various kinds of genetic interactions including 
suppression and synthetic lethality. Students will be immersed in yeast 
genomics and will perform and interpret experiments with DNA arrays. 
Students will gain first-hand experience in modern cytological
such as epitope tagging and imaging yeast cells using indirect 
immunofluorescence, GFP-protein fusions and a variety of fluorescent 
indicators for various subcellular organelles. Lectures on fundamental 
aspects of yeast genetics will be presented along with seminars given by 
outside speakers on topics of current interest. Last years speakers 
included: Jamie Konopka, State University of New York at Stony Brook, 
Angelika Amon, Massachusetts Institute of Technology, Anita Hopper, 
Pennsylvania State University, Bruce Futcher, Cold Spring Harbor 
Laboratory, Michael Lichten, National Cancer Institute, Ted Weinert, 
University of Arizona, David Roof, University of Pennsylvania, Pam
Sloan Kettering, David Pellman, Harvard University, Gerry Fink, 
Massachusetts Institute of Technology, Mike Snyder, Yale University,
Lundbald, Baylor College, Jeff Boeke, Johns Hopkins University, and Mark 
Winey, University of Colorado.
Cold Spring Harbor Course
Advanced Drosophila Genetics
July 31 - August 13
Michael Ashburner, University of Cambridge, United Kingdom
Scott Hawley, University of California, Davis
This intensive seminar course provides an introduction to the theory and 
practice of methods used to manipulate the Drosophila genome. It is 
suitable for graduate students and researchers with some experience with 
Drosophila who are interested in expanding their knowledge of the wide 
range of genetic techniques now available for use with this organism. 
Topics covered will include chromosome mechanics, the design and
of genetic screens, and the use of transposable elements as genetic
This year will be the year of the fly genome, and its impact on genetic 
analysis will be a particular focus of the course.

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