GFP purification short course

Daniel Gonzalez meton at rci.rutgers.edu
Wed Dec 4 22:11:34 EST 2002


Short Course in Protein Purification Using GFP

I have been asked to announce the following,

                  The State University of New Jersey
                  Rutgers (Campus at New Brunswick)

             Center for Research and Education in Bioluminescence
                          and Biotechnology
                   Programs in Biotechnology, Presents:

Protein Purification: Isolation, Analysis, and Characterization of GFP,
A Five and One-Half Day Hands-On Laboratory Course Using the remarkable
Green-Fluorescent Protein (GFP), A Novel Marker For Gene Expression,
as the source material;

                               January 12-17, 2003
                               and  March 16-21, 2003

More than 1100 scientists from around the world have strongly
recommended this intensive course as an opportunity to develop
protein research and analytical skills in a retreat setting.
Participants work hard, identify and solve problems in the lab and
enjoy camaraderie and good food and beer with colleagues.  An
added bonus of this course is practical laboratory exposure to the
GFP, now a staple of life sciences laboratories everywhere.

This five and one-half day laboratory course covers a wide variety
of conventional methods for protein isolation, purification, and
characterization.  The course format integrates hands-on laboratory
exercises with classroom lectures, demonstrations, study breaks,
and short take-home assignments.

A special feature of the course is that all laboratory work will be
performed on the same starting sample (native or recombinant
Aequorea GFP), which will be purified from an exceedingly crude
form to near homogeneity as judged by high performance liquid
chromatography (HPLC), SDS gel electrophoresis, and isoelectric
focusing. This feature provides a continuity of purpose, integrating
dozens of preparative and analytical protein techniques in a way
that few competing courses can match.

A problem-solving approach will be used throughout the course.  
Under the guidance of experienced lab instructors, participants
will work in groups of three to plan their own protocols, analyze
data, and interpret results. A student-teacher ratio not greater than
8:1 will be maintained and the faculty coordinators will be present
throughout the course.

For further details you can reach us,

by E-mail at:  meton at rci.rutgers.edu or crebb at rci.rutgers.edu
by phone at:  (732) 932-9071 extension 225 or 216
by FAX at:  (732) 932-3633

for a brochure and further information please visit the GFP
purification short course official Web site at:
http://www.rci.rutgers.edu/~meton/protein.html

For a lecture only  course which covers these topics, we present
"Biochemical Separations: An Introduction to Laboratory
techniques" --January 9-10, 2003.   For more info please visit
the web site for the course.
http://www.rci.rutgers.edu/~meton/biosep.html




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