In <9401141601.AA01744 at net.bio.net>
mroussel at alchemy.chem.utoronto.ca (Marc Roussel) writes:
>In article <2h4lf5$kuj at mserv1.dl.ac.uk> SCHLOSSER at ciit.org writes:
>>In <9401132045.AA17957 at net.bio.net>
>>mroussel at alchemy.chem.utoronto.ca (Marc Roussel) writes:
>>>I'm not so sure. I would like to do the calculations but I don't know
>>>how the glycerol is generated. (It would be sufficient for me to know
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
>>>the reactants and products of the reaction.) I take it that the ethanol
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
>>>is generated by anaerobic fermentation.
>>>Based on the measured fluxes, the stoichiometry is approximately:
>>>glucose -> 1.41(ethanol + CO2) + 0.24glycerol + "polysaccharides"
>> Unfortunately, it's impossible to do a proper equilibrium
>calculation with this sort of specification, at least using methods
>known to me. It's too vague. (Needless to say, I would be most
>interested in hearing about methods that make this tractable.)
The *only* reactant is glucose (well the inorganic phosphate comes on
and off and there are a few hydrogens picked up.) The primary products
are ethanol, CO2 and glycerol. Let's ignore the "polysaccharides" for
now, so the *only* products are ethanol, CO2 and glycerol, with the
following stoichiometry:
glucose + 0.29H2 = 1.71(ethanol + CO2) + 0.29glycerol
occurring at 20C. I can supply additionaly info, if you can let me
know what's needed (from the statement quoted above, I thought that this
was "sufficient".) The exact (intermediate) steps are those in the
standard glycolysis pathway.
Paul
schlosser at beta.ciit.org
