In article <2vgj0d$4q6 at mserv1.dl.ac.uk>, Jannie Hofmeyr
<jhsh at maties.sun.ac.za> wrote:
> Dear Netters
>> I am looking for a recent review of different techniques
> for permeabilising cells. The most recent one I could find was by Felix
> [Anal. Biochem. 120, 211-234, 1982]. I am specifically interested in
> permeabilising E. coli cells for the purpose of investigating serine
> biosynthesis (no leakage of proteins, permeable to metabolites up to a
> Mr of about 800, NAD(H), ATP, etc.).
>> Thanks!
> Jannie Hofmeyr
>> ========================================================================
> Jannie Hofmeyr InterNet e-mail: jhsh at maties.sun.ac.za> Dept. of Biochemistry Telephone: +27 21 8083039
> University of Stellenbosch Fax: +27 21 8083022
> Stellenbosch 7600
> South Africa
> ========================================================================
Two articles may be helpful.
1) Vaara, M. (1992) Agents that increase the permeability of the outer
membrane. Microbiol. Rev. 56:395-411
2) Hancock, R. E. W. (1984) Alterations in outer membrane permeability.
Ann. Rev. Microbiol. 38: 237-264
--
Ing-Nang Wang
ingnang at sbbiovm.sunysb.edu
Dept. Ecology and Evolution
SUNY-Stony Brook
