> I'd like to know likewise in cardiac myocytes. I would guess
> that NADH/NAD would be in equilibrium with mitochondrial NADH/NAD
> because of the malate-aspartate shuttle (I hope that I have the correct
> shuttle system). I saw a paper from a few years back by Balaban
> (Biophysical Journal?) measuring mitochondrial NADH/NAD ratio (no molar
> values) as 0.44. They used the fluorescence property of NADH.
> Interestingly, they saw no impact on fluorescence from increasing
> lactate concentration, which presumably would decrease cytosolic NADH
> via the lactate dehydrogenase reaction. Anyways, I know of no
> literature measuring actual molar values, the NADH/NAD is an indicator
> of redox state and has been tracked by those interested in the control
> of oxidative phosphorylation. Maybe, one of the authors of those papers
> (if you find them) could help you.
>> Richard Kondo
> Cellular Biophysics
> Boston University, Boston Massachusetts
Following up my own original posting, I have received a couple of e
-mails indicating an NAD/NADH ratio of 1000:1 in the cytoplasm. One person
suggested a ratio of 9:1 in mitochondria.
Roger C. Green, Faculty of Medicine Phone: (709)737-6884
Memorial University , St. John's, Newfoundland FAX : (709)737-7010
