In article <3qkrlq$jo1 at whitbeck.ncl.ac.uk>, Simon Eaton
<S.J.Eaton at ncl.ac.uk> wrote:
> kinase. I can't think of a way of regenerating ATP, apart from using
> pyuruvate kinase/ PEP, which regenerates ATP, but also eventually yields
> acetyl-CoA, which could confuse the issue of beta-oxidation itself.
Never worked with mitochondria, but for in vitro translation in
reticulocyte lysate (or brain extracts) we use creatine phosphate and
creatine phosphokinase (respectively 7 mM and 1 mg/ml) to regenerate ATP
from ADP.
--------------------------------------------------
Didier Gauthier
Dept. de chimie et biochimie
Universite de Moncton Fax: (506) 858-4355
Moncton, NB, E1A 3E9 E-mail: gauthid at umoncton.ca
Canada
