In article <3219AEC6.1592 at ibsm.cnrs-mrs.fr>, Athel <athel at IR2CBM.CNRS-
MRS.FR> writes
>We are interested in finding examples in the literature of clean
>experimental data showing rates of reactions catalysed by allosteric
>enzymes as a function of effector (inhibitor or activator)
>concentration at several different substrate concentrations. Although
>one might think that many such examples would exist in the literature
>from around 1970, we have had very little success in tracking them
>down. The classic paper of Blangy, Buc and Monod (1968) J. Molec.
>Biol. 31, 13-35 on phosphofructokinase is a good example of the sort
>of paper we are looking for, though it concentrates on rate as a
>function of substrate concentration rather than as a function of
>effector concentration. Another enzyme for which we have found data is
>aspartate transcarbamylase. References to papers on other enzymes will
>be very welcome.
>>It is not too difficult to find examples of the converse type of
>graph, i.e. plots of rate as a function of substrate concentration at
>two or three inhibitor or activator concentrations, but these are not
>really sufficient for the sort of analysis we would like to do.
>>Athel Cornish-Bowden (athel at ibsm.cnrs-mrs.fr)
>Jannie Hofmeyr (jhsh at maties.sun.ac.za)
>
I seem to remember some experiments of the sort you mention for Pyruvate
Kinase, but sorry can't remember the refs. Have to tried tracing
references using 'Enzyme Handbook'.
Strato
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