On 27 Sep 96 at 6:48, Nigel J.Osborn wrote:
> Does anyone know how to measure protein MW in the order of 500,000 to
> several million? Any help would be appreciated.
I am no expert on that, but I think that the best you'd get for that size
is multi-angle static light scattering. This would also give you the
hydrodynamic radius of the protein (the usual way to do this is by using
a Zimm plot, maybe you'll find interesting papers if you do a
bibliographic search for "Zimm plot").
Pedro Mendes Inst. Biological Sciences
prm at aber.ac.uk (home: pedro at enzyme.demon.co.uk) Univ. Wales, Aberystwyth,
http://gepasi.dbs.aber.ac.uk/pedro/prmhome.htm Dyfed SY23 3DA, U.K.
PGP mail welcome! (public key on demand) FAX: +44 (0)1970 622350
