IUBio

Very high MW determination?

Achim Recktenwald, PhD achim at ibex.ca
Fri Sep 27 08:24:45 EST 1996


Nigel J.Osborn wrote:
> 
> Does anyone know how to measure protein MW in the order of 500,000 to
> several million? Any help would be appreciated.
> 
> Nigel.


Depends how exact you need it. 
You could try gel-filtration with gels having pore sizes large enough
for viruses.
I would try Sepharose 2B or CL-2B.

Achim



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